Small-angle neutron and dynamic light scattering study of gelatin coacervates

Pramana

B Mohanty²  H B Bohidar1²  P S Goyal¹  V K Aswal¹ 

[1] Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India$$;School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110 067, India$$

关键词: Gelatin;    coacervates;    light scattering;    neutron scattering.;   

DOI  :  

学科分类：物理（综合）

来源: Indian Academy of Sciences

【 摘 要 】

The state of intermolecular aggregates and that of folded gelatin molecules could be characterized by dynamic laser light and small-angle neutron scattering experiments, which implied spontaneous segregation of particle sizes preceding coacervation, which is a liquid-liquid phase transition phenomenon. Dynamic light scattering (DLS) data analysis revealed two particle sizes until precipitation was reached. The smaller particles having a diameter of ∼ 50 nm (stable nanoparticles prepared by coacervation method) were detected in the supernatant, whereas the inter-molecular aggregates having a diameter of ∼ 400 nm gave rise to coacervation. Small-angle neutron scattering (SANS) experiments revealed that typical mesh size of the networks exist in polymer dense phase (coacervates) [1]. Analysis of the SANS structure factor showed the presence of two length scales associated with this system that were identified as the correlation length or mesh size, 𝜉 = 10.6 Å of the network and the other is the size of inhomogeneities = 21.4 Å. Observations were discussed based on the results obtained from SANS experiments performed in 5% (w/v) gelatin solution at 60° C (𝜉 = 50$ Å, 𝜁 = 113 Å) and 5% (w/v) gel at 28° C (𝜉 = 47 Å, 𝜁 = 115 Å) in aqueous phase [2] indicating smaller length scales in coacervate as compared to sol and gel.

【 授权许可】

Unknown