Collagen I-induced dendritic cells activation is regulated by TNF-𝛼 production through down-regulation of IRF4

Journal of biosciences

Hyeon-Hui Ki¹  Dae-Ki Kim1¹  Barun Poudel¹  Young-Mi Lee2² 

[1] Department of Immunology and Institute of Medical Sciences, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Korea$$;Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Korean Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 570-749, Korea$$

关键词: Anti-TNF-𝛼;    collagen I;    interleukin-12;    IRF4;    maturation;    monocyte-derived dendritic cells;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Previously we have shown that collagen I enhances the maturation and function of dendritic cells (DCs). Inflammatory mediators such as tumour necrosis factor (TNF)-𝛼, interleukin (IL)-1𝛽 and lipopolysaccharide (LPS) are also known to activate DCs. Here we investigated the involvement of TNF-𝛼 on the collagen I-induced DCs activation. TNF-a neutralization inhibited collagen I-induced IL-12 secretions by DCs. Additionally, we observed suppression of collagen I-induced costimulatory molecules expression along with down-regulation of genes involved in DCs activation pathway. Furthermore, TNF-𝛼 inhibition upon collagen Istimulation up-regulated the expression of interferon regulatory transcription factor IRF4, when compared to collagen I only treated cells. Collectively, our data demonstrate that collagen I induce TNF-𝛼 production, which is crucial for the activation and function of DCs, through down-regulation of IRF4, and implicates the importance in development of anti- TNF-𝛼 therapeutics for several inflammatory diseases.

【 授权许可】

Unknown