Effects of nitric oxide modulating activities on development of enteric nervous system mediated gut motility in chick embryo model

Journal of biosciences

Seyed-Muhammad Faghihi³  Ahad Muhammadnejad²  Hossein-Ali Arab1³  Samad Muhammadnejad³  Hossein Hassanpour¹ 

[1] Department of Basic Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran$$;Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran$$;Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran$$

关键词: Chicken embryo;    development;    enteric nervous system;    nitric oxide;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The enteric nervous system (ENS) arises from the enteric neural crest-derived cells (ENCCs), and many molecules and biochemical processes may be involved in its development. This study examined the effects of modulating embryonic nitric oxide (NO) activity on the intestinal motility induced by ENS. One-hundred-and-twenty fertilized chicken eggs were assigned to three main groups and incubated at 37°C and 60% humidity. The eggs were treated with 𝑁𝐺-nitro-L-arginine methyl ester (L-NAME), sodium nitroprusside (SNP), L-arginine (L-Arg) or vehicle from days 3 (1st group), 7 (2nd group) and 10 (3rd group) of incubation and continued up to day 18. On day 19, the embryos were sacrificed, the jejunal and colorectal segments were taken and the intestinal motility was assessed using isolated organ system. The intestinal motility was recorded normally and following cholinergic, adrenergic and non-adrenergic non-cholinergic (NANC) stimulations. The ENS structure was assessed by immunohistochemistry (IHC) using glial fibrillary acidic protein (GFAP). Rhythmic intestinal contractions were seen in all treatment groups, but inhibition of NO in the L-NAME-treated embryos caused significant decrease (𝑝 < 0.01) in the frequency and amplitude of the contraction. The responsiveness to adrenergic, cholinergic and NANC stimulations was also significantly decreased (𝑝 <0.05). The GFAP expression was significantly (𝑝 < 0.05) reduced in the L-NAME-treated embryos. This study showed that the inhibition of NO caused a deficient development of the ENS, leading to a decrease in the frequency and amplitude of the intestinal contractions and reduced the responsiveness to adrenergic, cholinergic and NANC signalling.

【 授权许可】

Unknown