Identification of chikungunya virus interacting proteins in mammalian cells

Journal of biosciences

Mandar S Paingankar¹  Vidya A Arankalle1¹ 

[1] National Institute of Virology, Microbial Containment Complex, 130/1 Sus Road, Pashan, Pune 411 021, India$$

关键词: Actin;    HSP70;    STAT-2;    virus overlay protein binding assay;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Identification and characterization of virus host interactions is an essential step for the development of novel antiviral strategies. Very few studies have been targeted towards identification of chikungunya virus (CHIKV) interacting host proteins. In current study, virus overlay protein binding assay (VOPBA) and matrix-assisted laser desorption/ionization time of flight analysis (MALDI TOF/TOF) were employed for the identification of CHIKV binding proteins in mammalian cells. HSP70 and actin were identified as virus binding proteins in HEK-293T and Vero-E6 cells, whereas STAT-2 was identified as an additional protein in Vero-E6 cells. Pre-incubation with anti-HSP70 antibody and miRNA silencing of HSP70 significantly reduced the CHIKV production in HEK-293T and Vero-E6 cells at early time points. These results suggest that CHIKV exploits the housekeeping molecules such as actin, HSP70 and STAT-2 to establish infection in the mammalian cells.

【 授权许可】

Unknown