Occurrence and molecular characterization of Cryptosporidium in dogs in Henan Province, China

BMC Veterinary Research

Longxian Zhang²  Heping Dong¹  Sumei Zhang²  Rongjun Wang²  Xiaoyi He³  Meng Qi²  Fuchun Jian² 

[1] College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, P R, China;International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, P R, China;Xiangya School of Medicine, Central South University, Changsha 410013, P R, China

关键词: Actin;    HSP70;    SSU rRNA;    Cryptosporidium canis;    Dogs;    Infection rate;   

Others  :  1119344
DOI  :  10.1186/1746-6148-10-26

 received in 2013-08-07, accepted in 2014-01-15,  发布年份 2014

【 摘 要 】

Background

Cryptosporidiosis in dogs has been reported worldwide, involving both asymptomatic and diarrheic dogs. Large-scale surveys of Cryptosporidium infection in dogs have been performed in some countries using differents diagnostic methods. But, few data are available on the infection rate and molecular characteristics of Cryptosporidium spp. in dogs in China.

Result

In this study, 770 fecal samples from 66 locations in Henan Province were examined. The average Cryptosporidium infection rate was 3.8%, with dogs in kennels having the highest rate of 7.0% (χ² = 14.82, P < 0.01). The infection rate was 8.0% in dogs younger than 90 days, which was significantly higher than that in the other age groups (1.1–3.8%;χ² = 18.82, P < 0.01). No association was noted between the infection rate and the sex of the dogs. Twenty-nine Cryptosporidium-positive samples were amplified at the small subunit rRNA (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin loci using PCR. Sequence analysis of these amplicons identified only Cryptosporidium canis, which showed 100% identity with the published sequences of the SSU rRNA, HSP70, and actin genes.

Conclusions

Our results confirm that C. canis is popular in the dog population in China, considering the large number of dogs in China and the close contact between dogs and humans, the role of C. canis in the transmission of human cryptosporidiosis warrants attention.

【 授权许可】

   
2014 Jian et al.; licensee BioMed Central Ltd.

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