MiR-200a enhances the migrations of A549 and SK-MES-1 cells by regulating the expression of TSPAN1

Journal of biosciences

Wei Peng²  Yixiang Lu²  Jianxin Chen²  Yaqing Chen¹  Tao Xi1¹  York Yuanyuan Zhu2² 

[1] School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China$$;Biomics Biotechnologies Co. Ltd, Nantong, 226016, China$$

关键词: MicroRNA;    migration;    miR-200a;    NSCLC;    TSPAN1;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

MicroRNA-200a (miR-200a) has been reported to regulate tumour progression in several tumours; however, little is known about its role in non-small cell lung cancer cells (NSCLCs). Here, we found that miR-200a was up-regulated in A549 and SK-MES-1 cells compared with normal lung cells HELF. By a series of gain-of-function and loss-offunction studies, over-expression of miR-200a was indicated to enhance cells migration, and its knock-down inhibited migration of cells in NSCLC cell lines. Furthermore, miR-200a was identified to induce TSPAN1 expression which was related to migration. TSPAN1 was proved to induce migration, and so up-regulation of TSPAN1 by miR-200a may explain why over-expressing miR-200a promotes NSCLC cells migration.

【 授权许可】

Unknown