Characterization of P1 promoter activity of the 𝛽-galactoside 𝛼2,6-sialyltransferase I gene (siat 1) in cervical and hepatic cancer cell lines

Journal of biosciences

Lorena Milflores-Flores¹  Gerardo Santos-López¹  Julio Reyes-Leyva¹  Verónica Vallejo-Ruiz1¹  Lourdes Millán-Pérez² 

[1] Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Hospital General de Zona No. 5, Km 4.5 Carretera Federal Atlixco-Metepec, 74360 Metepec, Puebla, México$$;Centro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, México$$

关键词: Cancer cells;    𝛽-galactoside 𝛼2;    6-sialyltransferase I;    promoter;    siat 1 gene;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The level of 𝛽-galactoside 𝛼2,6-sialyltransferase I (ST6Gal I) mRNA, encoded by the gene siat1, is increased in malignant tissues. Expression is regulated by different promoters – P1, P2 and P3 – generating three mRNA isoforms H, X and YZ. In cervical cancer tissue the mRNA isoform H, which results from P1 promoter activity, is increased. To study the regulation of P1 promoter, different constructs from P1 promoter were evaluated by luciferase assays in cervical and hepatic cell lines. Deletion of a fragment of 1048 bp (−89 to +24 bp) increased 5- and 3-fold the promoter activity in C33A and HepG2 cell lines, respectively. The minimal region with promoter activity was a 37 bp fragment in C33A cells. The activity of this region does not require the presence of an initiator sequence. In HepG2 cells the minimal promoter activity was detected in the 66 bp fragment. Sp1 (−32) mutation increased the promoter activity only in HepG2 cells. HNF1 mutation decreased promoter activity in HepG2 cell line but not in C33A cells. We identified a large region that plays a negative regulation role. The regulation of promoter activity is cell type specific. Our study provides new insights into the complex transcriptional regulation of siat1 gene.

【 授权许可】

Unknown