Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus

Journal of biosciences

João C Deschamps²  Alan John A McBride²  Fabiana K Seixas²  Luis Fernando Marins¹  Vinicius F Campos1²  Luís A Sampaio³  Marcelo Okamoto³  Tiago Collares²  Ricardo B Robaldo⁴ 

[1] Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, CP 474, 96201-900, Rio Grande, RS, Brazil$$;Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, CP 354, 96010-900, Pelotas, RS, Brazil$$;Laboratório de Piscicultura Estuarina e Marinha, Instituto de Oceanografia, Universidade Federal do Rio Grande, CP 474, 96201-900, Rio Grande, RS, Brazil$$;Departamento de Fisiologia e Farmacologia, Instituto de Biologia, Universidade Federal de Pelotas, CP 354, 96010-900, Pelotas, RS, Brazil$$

关键词: Food intake;    mRNA expression;    NPY;    Paralichthys orbignyanus;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Neuropeptide Y (NPY) is considered the major stimulant for food intake in mammals and fish. Previous results indicate that NPY is involved in the feeding behaviour of the Brazilian flounder, Paralichthys orbignyanus. In this study, we evaluated hypothalamic NPY expression before (−2 h), during (0 h) and after feeding (+2 h) in two independent experiments: (1) during a normal feeding schedule and (2) in fish fasted for 2 weeks. During normal feeding, changes in the levels of NPY mRNA were periprandial, with expression levels being significantly elevated at meal time (𝑃 < 0.05) and significantly reduced 2 h later (𝑃< 0.05). Comparing the fasting and unfasted groups, NPY mRNA levels were significantly higher (𝑃 < 0.05) at −2 h and +2 h in the fasting group, but there was no difference at 0 h. In addition, the higher NPY mRNA levels that were observed in the fasting group were maintained throughout the sampling period. In summary, our results show that NPY expression was associated with meal time (0 h) in food intake regulation.

【 授权许可】

Unknown