Journal of biosciences | |
Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus | |
João C Deschamps2  Alan John A McBride2  Fabiana K Seixas2  Luis Fernando Marins1  Vinicius F Campos12  LuÃs A Sampaio3  Marcelo Okamoto3  Tiago Collares2  Ricardo B Robaldo4  | |
[1] Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, CP 474, 96201-900, Rio Grande, RS, Brazil$$;Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, CP 354, 96010-900, Pelotas, RS, Brazil$$;Laboratório de Piscicultura Estuarina e Marinha, Instituto de Oceanografia, Universidade Federal do Rio Grande, CP 474, 96201-900, Rio Grande, RS, Brazil$$;Departamento de Fisiologia e Farmacologia, Instituto de Biologia, Universidade Federal de Pelotas, CP 354, 96010-900, Pelotas, RS, Brazil$$ | |
关键词: Food intake; mRNA expression; NPY; Paralichthys orbignyanus; | |
DOI : | |
来源: Indian Academy of Sciences | |
【 摘 要 】
Neuropeptide Y (NPY) is considered the major stimulant for food intake in mammals and fish. Previous results indicate that NPY is involved in the feeding behaviour of the Brazilian flounder, Paralichthys orbignyanus. In this study, we evaluated hypothalamic NPY expression before (−2 h), during (0 h) and after feeding (+2 h) in two independent experiments: (1) during a normal feeding schedule and (2) in fish fasted for 2 weeks. During normal feeding, changes in the levels of NPY mRNA were periprandial, with expression levels being significantly elevated at meal time (𑃠< 0.05) and significantly reduced 2 h later (ð‘ƒ< 0.05). Comparing the fasting and unfasted groups, NPY mRNA levels were significantly higher (𑃠< 0.05) at −2 h and +2 h in the fasting group, but there was no difference at 0 h. In addition, the higher NPY mRNA levels that were observed in the fasting group were maintained throughout the sampling period. In summary, our results show that NPY expression was associated with meal time (0 h) in food intake regulation.
【 授权许可】
Unknown
【 预 览 】
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RO201912040495091ZK.pdf | 134KB | download |