Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus

Journal of biosciences

Carlos Frederico C Lanes²  João C Deschamps³  Fabiana K Seixas³  Luis Fernando Marins⁵  Odir A Dellagostin³  Juliana Sandrini⁵  Vinicius F Campos1³  Luís A Sampaio¹  Marcelo Okamoto¹  Tiago Collares³  Ricardo B Robaldo⁴ 

[1] Laboratório de Piscicultura Estuarina e Marinha, Instituto de Oceanografia, Universidade Federal do Rio Grande, CP 474, 96201-900, Rio Grande, RS, Brazil$$;Faculty of Biosciences and Aquaculture, Bodø University College, NO-8049 Bodø, Norway$$;Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, CP 354, 96010-900, Pelotas, RS, Brazil$$;Departamento de Fisiologia e Farmacologia, Instituto de Biologia, Universidade Federal de Pelotas, CP 354, 96010-900, Pelotas, RS, Brazil$$;Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, CP 474, 96201-900, Rio Grande, RS, Brazil$$

关键词: Food intake;    gene expression;    NPY;    Paralichthys orbignyanus;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Neuropeptide Y (NPY) is one of the most potent stimulants of food intake in vertebrates, mammals and fish. However, the present knowledge about feeding behaviour in fish is still limited and based on studies in a few species. The Brazilian flounder Paralichthys orbignyanus is being considered for aquaculture, and it is important to understand the mechanisms regulating feeding in order to improve its performance in captivity. The objectives of this study were to clone NPY cDNA, evaluate the mRNA levels in different tissues of flounder, and also evaluate brain NPY expression to associate food intake with NPY expression levels. A 597 bp NPY cDNA was cloned from Brazilian flounder brain. NPY expression was detected in all the peripheral tissues analysed. No significant differences were observed in brain NPY gene expression over 24 h after food intake at a temperature of 15 ± 3°C. No correlation was observed among plasma glucose, total protein, cholesterol, triglycerides and NPY expression levels during this 24 h period. On the other hand, mRNA levels were increased after two weeks of fasting at elevated temperatures. Our results suggest that NPY mRNA levels in Brazilian flounder are affected by temperature.

【 授权许可】

Unknown