Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat

Journal of biosciences

Rui-Lian Jing1¹  Qing-Wei Zang¹  Xiao-Ping Chang¹  Xu-Yan Li¹  Jun Zhao²  Cai-Xiang Wang¹  Zhi-Ai Guo¹ 

[1] The National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm & Biotechnology, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China$$;Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China$$

关键词: Cysteine protease;    TaCP;    water deficit tolerance;    gene expression;    common wheat;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Plant cysteine protease (CP) genes are induced by abiotic stresses such as drought, yet their functions remain largely unknown. We isolated the full-length cDNA encoding a Triticum aestivum CP gene, designated TaCP, from wheat by the rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that TaCP contains an open reading frame encoding a protein of 362 amino acids, which is 96% identical to barley cysteine protease HvSF42. The TaCP transcript level in wheat seedlings was upregulated during polyethylene glycol (PEG) stress, with a peak appearing around 12 h after treatment. TaCP expression level increased rapidly with NaCl treatment at 48 h. TaCP responded strongly to low temperature (4°C) treatment from 1 h post-treatment and reached a peak of about 40-fold at 72 h. However, it showed only a very slight response to abscisic acid (ABA). More than one copy of TaCP was present in each of the three genomes of hexaploid wheat and its diploid donors. TaCP fused with green fluorescent protein (GFP) was located in the plasma membrane of onion epidermis cells. Transgenic Arabidopsis plants overexpressing TaCP showed stronger drought tolerance and higher CP activity under water-stressed conditions than wild-type Arabidopsis plants. The results suggest that TaCP plays a role in tolerance to water deficit.

【 授权许可】

Unknown