On the conspecificity of Anopheles fluviatilis species S with Anopheles minimus species C

Journal of biosciences

T Adak⁴  D Chandra⁴  O P Singh1⁴  Pe Than Htun²  S K Sharma¹  S K Subbarao³  A P Dash⁴  N Nanda⁴ 

[1] National Institute of Malaria Research, Field Station, Sector 5, Rourkela 769 002, India$$;Department of Medical Research, 5 Ziwaka Road, Dagon Township 11191, Yangan, Myanmar$$;Indian Council of Medical Research, Ansari Nagar, New Delhi 110 029, India$$;National Institute of Malaria Research, 22 Sham Nath Marg, Delhi 110 054, India$$

关键词: Anopheles fluviatilis;    Anopheles minimus;    malaria;    ribosomal DNA;    molecular taxonomy;    sibling species;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Anopheles fluviatilis and An. minimus complexes, each comprising of at least three sibling species, are closely related and important malaria vectors in Oriental Region. Recently An. fluviatilis species S, which is a highly efficient malaria vector in India, has been made conspecific with An. minimus species C (senior synonym) on the basis of homology in 335 base pair nucleotide sequence of D3 domain of 28S ribosomal DNA (rDNA). We examined the conspecificity of these two nominal species by obtaining and analysing the DNA sequences of nuclear ribosomal loci internal transcribed spacer 2 (ITS2) and D2-D3 domain of 28S rDNA (28S-D2/D3) from those of An. fluviatilis S and An. minimus C. We found that the sequences of An. fluviatilis S are appreciably different from those of An. minimus C with pair-wise distance (Kimura-2-parametre model) of 3.6 and 0.7% for loci ITS2 and 28S-D2/D3, respectively. Pair-wise distance and phylogenetic analyses using ITS2 sequences of members of Minimus and Fluviatilis Complexes revealed that An. fluviatilis S is distantly related to An. minimus C as compared to any other members of the Fluviatilis Complex. These findings suggest that the two nominal species, An. fluviatilis S and An. minimus C, do not merit synonymy. The study also confirms that the reported species An. fluviatilis X is synonym with species S.

【 授权许可】

Unknown