Aujeszky's disease virus production in disposable bioreactor

Journal of biosciences

K RadoÅ¡ević¹  V Gaurina SrÄ?ek¹  I Slivac¹  I KmetiÄ?²  Z Kniewald1¹ 

[1] Laboratory for Cell Culture Technology and Biotransformations, University of Zagreb, 6 Pierotti St., HR-10000 Zagreb, Croatia$$;Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, 6 Pierotti St., HR-10000 Zagreb, Croatia$$

关键词: Aujeszky's disease virus;    baby hamster kidney cells;    cell culture;    disposable bioreactor;    virus titre;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

A novel, disposable-bag bioreactor system that uses wave action for mixing and transferring oxygen was evaluated for BHK 21 C13 cell line growth and Aujeszky’s disease virus (ADV) production. Growth kinetics of BHK 21 C13 cells in the wave bioreactor during 3-day period were determined. At the end of the 3-day culture period and cell density of 1.82 × 106 cells ml–1, the reactor was inoculated with 9 ml of gE- Bartha K-61 strain ADV suspension (105.9 TCID50) with multiplicity of infection (MOI) of 0.01. After a 144 h incubation period, 400 ml of ADV harvest was obtained with titre of 107.0 TCID50 ml–1, which corresponds to 40,000 doses of vaccine against AD. In conclusion, the results obtained with the wave bioreactor using BHK 21 C13 cells showed that this system can be considered as suitable for ADV or BHK 21 C13 cell biomass production.

【 授权许可】

Unknown