Journal of biosciences | |
Ni2+ -uptake in Pseudomonas putida strain S4: a possible role of Mg2+ -uptake pump | |
V N Tripathi1  S Srivastava11  | |
[1] Department of Genetics, University of Delhi, South Campus, New Delhi 110 021, India$$ | |
关键词: CorA; Co(III)Hex; metal uptake; Mg2+; Ni2+; | |
DOI : | |
来源: Indian Academy of Sciences | |
【 摘 要 】
Essential metal ion homeostasis is based on regulated uptake of metal ions, both during its scarcity and abundance. Pseudomonas putida strain S4, a multimetal resistant bacterium, was employed to investigate Ni2+ entry into cells. It was observed that Mg2+ regulates the entry of Ni2+ and by this plays a protective role to minimize Ni2+ toxicity in this strain. This protection was evident in both growth as well as viability. Intracellular accumulation of Ni2+ varied in accordance with Mg2+ concentrations in the medium. It was hypothesized that Ni2+ enters the cell using a broad Mg2+ pump, i.e. the CorA system, as the CorA inhibitor, i.e. Co(III) Hex, also inhibits Ni2+ uptake. This led to the inference that Mg2+-based protection was basically due to competitive inhibition of Ni2+ uptake. We also show that Zn2+ can further regulate the entry of Ni2+.
【 授权许可】
Unknown
【 预 览 】
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RO201912040494485ZK.pdf | 365KB | download |