An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton

Journal of biosciences

Yingfan Cai¹  Fang Yan²  Yu Zeng²  Jie Bai²  Chen Miao²  Fang Chen2²  Hong Zhang²  Jianchuan Mo¹  Jinku Bao² 

[1] College of Bioinformation, Chongqing University of Post and Telecom, Chongqing 400065, People’s Republic of China$$;College of Life Science, Sichuan University, Chengdu 610064, People’s Republic of China$$

关键词: Cotton (Gossypium);    gossypol;    high-performance liquid chromatography (HPLC);    pigment gland;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (HPLC) on a C18 column (4.6 mm × 250 mm, 5 𝜇m particle) with methanol–0.5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] < 10%, both intra-day and inter-day. Absolute recoveries were > 94%. The results revealed major differences among the different gland varieties or species of cotton, including the special and ordinary glandless and glanded Gossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton.

【 授权许可】

Unknown