Tropane alkaloids of Atropa belladonna L. and Atropa acuminata Royle ex Miers plants

Dynamic Chiropractic

Fatemeh Ashtiania¹  Fatemeh Sefidkonb² 

[1] Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.;Research Institute of Forests and Rangelands, P.O. Box: 13185-116, Tehran, Iran.

关键词: Atropa belladonnaL.;    Atropa acuminataRoyle ex Miers;    high-performance liquid chromatography (HPLC);    Tropane alkaloids.;   

DOI  :  10.5897/JMPR11.482

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The purpose of the present study was to determine the tropane alkaloid content in different parts of the wild and cultivatedAtropa belladonnaL. and wildAtropa acuminataRoyle ex Miers in Iran. Determination of alkaloids was performed by high-performance liquid chromatography (HPLC) method. Samples were extracted with chloroform- methanol- cc. ammonia 15:15:1(v/v/v). HPLC separation was performed on two C8columns. An isocratic mobile phase of acetonitrile- 50 mM phosphate buffer (pH 2.95) 10:90 and 20:80(v/v) was used. Peaks were identified by standards and diode-array detection. Scopolamine and atropine were determined by external method at 210 nm. The total alkaloid content of theA. belladonnaL.of sari-kiassar road was determined by a HPLC method to be 2.88% for the leaves and 8.06% for the roots and 1.42% for the stem. The total alkaloid content of the cultivatedA. belladonnaL.was 1.76% for the leaves, 3.3% for the roots, 1.42% for the stem and 4.82% for the seeds. Total alkaloid content of the leaves ofA. acuminatawas 5.88%. In all samples the percentage of atropine was higher than scopolamine. The results showed thatA. belladonnaandA. acuminataconstitutes the major storage site and had the highest amount of these alkaloids. These alkaloids may be transported to other plant organs providing potential deterrent and antimicrobial activities.

【 授权许可】

Unknown