Sample size for collecting germplasms – a polyploid model with mixed mating system

Journal of biosciences

R L Sapra1¹  Prem Narain¹  S K Lal¹  S V S Chauhan²  B B Singh¹ 

[1] Genetics Division, Indian Agricultural Research Institute, Pusa, New Delhi 110 012, India B-3/27A, Lawrence Road, New Delhi 110 035, India$$;Department of Botany, School of Life Sciences, BR Ambedkar University, Agra 282 002, India$$

关键词: Conservation;    diploid;    exploration;    germplasm;    inbreeding;    polyploid;    seeds;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The present paper discusses a general expression for determining the minimum sample size (plants) for a given number of seeds or vice versa for capturing multiple allelic diversity. The model considers sampling from a large 2 k-ploid population under a broad range of mating systems. Numerous expressions/results developed for germplasm collection/regeneration for diploid populations by earlier workers can be directly deduced from our general expression by assigning appropriate values of the corresponding parameters. A seed factor which influences the plant sample size has also been isolated to aid the collectors in selecting the appropriate combination of number of plants and seeds per plant. When genotypic multiplicity of seeds is taken into consideration, a sample size of even less than 172 plants can conserve diversity of 20 alleles from 50,000 polymorphic loci with a very large probability of conservation (0.9999) in most of the cases.

【 授权许可】

Unknown