Alterations in tumour suppressor gene p53 in human gliomas from Indian patients

Journal of biosciences

Kumaravel Somasundaram1¹  S Kalai Selvi¹  A S Hegde²  Pornima Phatak¹  Sridevi Hegde²  T Divya² 

[1] Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India$$;Manipal Institute for Neurological Disorders, Manipal Hospital, Airport Road, Bangalore 560 017, India$$

关键词: Gliomas;    mutations;    p53;    single strand conformation polymorphism;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Alterations in the tumour suppressor p53 gene are among the most common defects seen in a variety of human cancers. In order to study the significance of the p53 gene in the genesis and development of human glioma from Indian patients, we checked 44 untreated primary gliomas for mutations in exons 5–9 of the p53 gene by PCR-SSCP and DNA sequencing. Sequencing analysis revealed six missense mutations. The incidence of p53 mutations was 13.6% (6 of 44). All the six mutations were found to be located in the central core domain of p53, which carries the sequence-specific DNA-binding domain. These results suggest a rather low incidence but a definite involvement of p53 mutations in the gliomas of Indian patients.

【 授权许可】

Unknown