Gas chromatographic and mass spectrometric analysis of conjugated steroids in urine

Journal of biosciences

Kyung Ho Lee¹  Jong Man Yoon1² 

[1] Department of Environmental Engineering, Kyongju University, Kyongju City, Kyongsangbuk-do, 780-210, Korea$$;Department of Marine Biomedical Science, College of Ocean Science and Technology, Kunsan National University, Kunsan City, Jeollabuk-do, 573-702, Korea$$

关键词: Conjugated steroids;    gas chromatography;    mass spectrometry;    metabolite;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

This study was carried out qualitatively and quantitatively to investigate the presence and the concentrations of anabolic steroids in urine collected from orally administered humans. Microanalysis of conjugated steroids by gas chromatography and mass spectrometry (GC/MS) has been carried out. Following oral administration three major metabolites of anabolic steroid drugs have been detected and partially characterized. The six steroids can be analysed at the same time in 17 min. The lower detection limit was 10 ng/ml in 5 ml of urine. The conjugated steroids from urine were centrifuged to 2,430 𝑔 for 10 min, the supernatant solution passed through Amberlite XAD-2 column and the steroids eluted fraction esterified by using MSTFA and TMSI. The rate of metabolism and urinary excretion seem to be reasonably fast.

【 授权许可】

Unknown