Journal of biosciences | |
Analysis of BRCA1 involvement in breast cancer in Indian women | |
I Mittra11  P H Pestonjamasp1  | |
[1] Division of Laboratory Medicine, Department of Surgery, Tata Memorial Hospital, Ernest Borges Road, Parel, Mumbai 400 012, India$$ | |
关键词: BRCA1; breast cancer; microsatellite polymorphisms; tumour suppressive function; | |
DOI : | |
来源: Indian Academy of Sciences | |
【 摘 要 】
The involvement of the familial breast-ovarian cancer gene (BRCA1) in the molecular pathogenesis of breast cancer among Indian women is unknown. We have used a set of microsatellite polymorphisms to examine the frequency of allele loss at the BRCA1 region on chromosome 17q21, in a panel of 80 human breast tumours. Tumour and blood leukocyte/normal tissue DNA from a series of 80 patients with primary breast cancer was screened by PCR-amplified microsatellite length polymorphisms to detect deletions at three polymorphic BRCA1 loci. PCR-allelotype was valuable in examining allele losses from archival and small tumour samples. Loss of alleles at BRCA1 in the patient set, confirmed a noteworthy role of this gene in the molecular pathogenesis of breast cancer and was in accordance with its well-documented tumour suppressive function.
【 授权许可】
Unknown
【 预 览 】
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RO201912040493988ZK.pdf | 36KB | download |