Analysis of BRCA1 involvement in breast cancer in Indian women

Journal of biosciences

I Mittra1¹  P H Pestonjamasp¹ 

[1] Division of Laboratory Medicine, Department of Surgery, Tata Memorial Hospital, Ernest Borges Road, Parel, Mumbai 400 012, India$$

关键词: BRCA1;    breast cancer;    microsatellite polymorphisms;    tumour suppressive function;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The involvement of the familial breast-ovarian cancer gene (BRCA1) in the molecular pathogenesis of breast cancer among Indian women is unknown. We have used a set of microsatellite polymorphisms to examine the frequency of allele loss at the BRCA1 region on chromosome 17q21, in a panel of 80 human breast tumours. Tumour and blood leukocyte/normal tissue DNA from a series of 80 patients with primary breast cancer was screened by PCR-amplified microsatellite length polymorphisms to detect deletions at three polymorphic BRCA1 loci. PCR-allelotype was valuable in examining allele losses from archival and small tumour samples. Loss of alleles at BRCA1 in the patient set, confirmed a noteworthy role of this gene in the molecular pathogenesis of breast cancer and was in accordance with its well-documented tumour suppressive function.

【 授权许可】

Unknown