Complement component 3: characterization and association with mastitis resistance in Egyptian water buffalo and cattle

Journal of genetics

LAMEES HEGAZY⁴  NERMIN EL-HALAWANY1²  YASSER A. GHAZI³  DESSIE SALILEW-WONDIM⁵  HAMDY ABDEL-SHAFY¹  MAGDY A. ABDEL-LATIF⁶  AHMED F. M. AL-TOHAMY²  ABD-EL-MONSIF A. SHAWKY²  CHRISTIANE NEUHOFF⁵  KARL SCHELLANDER⁵ 

[1] Faculty of Agriculture, Department of Animal Production, Cairo University, 12613 Giza, Egypt$$;Department of Cell Biology, National Research Centre, 12622 Giza, Egypt$$;Department of Animal Reproduction and A.I. National Research Centre, 12622 Giza, Egypt$$;Avisina Group, Benha, Qalubia 13776, Egypt$$;Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn 53115, Germany$$;Department of Buffalo Research, Animal Production Research Institute, Doki, 12126 Giza, Egypt$$

关键词: complement component C3;    genetic variants;    somatic cell score;    association analysis;    buffalo;    cattle.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Mastitis is an infectious disease of the mammary gland that leads to reduced milk production and change in milk composition. Complement component C3 plays a major role as a central molecule of the complement cascade involving in killing ofmicroorganisms, either directly or in cooperation with phagocytic cells. C3 cDNA were isolated, from Egyptian buffalo and cattle, sequenced and characterized. The C3 cDNA sequences of buffalo and cattle consist of 5025 and 5019 bp, respectively. Buffalo and cattle C3 cDNAs share 99% of sequence identity with each other. The 4986 bp open reading frame in buffalo encodes a putative protein of 1661 amino acids—as in cattle—and includes all the functional domains. Further, analysis of the C3 cDNA sequences detected six novel single-nucleotide polymorphisms (SNPs) in buffalo and three novel SNPs in cattle.The association analysis of the detected SNPs with milk somatic cell score as an indicator of mastitis revealed that the most significant association in buffalo was found in the C>A substitution (ss: 1752816097) in exon 27, whereas in cattle it was in the C>T substitution (ss: 1752816085) in exon 12. Our findings provide preliminary information about the contribution of C3 polymorphisms to mastitis resistance in buffalo and cattle.

【 授权许可】

Unknown