Cytological behaviour of floral organs andin silico characterization of differentially expressed transcript-derived fragments associated with ‘floral bud distortion’ in soybean

Journal of genetics

R. S. NANDANWAR⁵  PRAVIN V. JADHAV1⁶  M. P. MOHARIL⁶  J. G. MANJAYA³  M. S. DUDHARE¹  S. S. MANE⁴  A. G. DESHMUKH²  RACHANA S. WAKEKAR⁶  PRASHANT B. KALE⁶  R. G. DANI⁶ 

[1] Vasantrao Naik College of Agricultural Biotechnology, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Yavatmal 445 001, India$$;Nagarjuna Medicinal Garden,Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola 444 104, India$$;Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre (BARC), Trombay, Mumbai 400 085, India$$;Department of Plant Pathology, Post Graduate Institute, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola 444 104, India$$;Department of Agricultural Botany,Post Graduate Institute, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola 444 104, India$$;Biotechnology Centre,Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola 444 104, India$$

关键词: soybean;    floral bud distortion;    cDNA-decamer;    cytology;    in silico characterization;    annotation;    mapping.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

An attempt was made to understand the ‘floral bud distortion’ (FBD), an unexplored disorder prevailing in soybean. Cytological behaviour of floral reproductive organs and in silico characterization of differentially expressed transcript-derivedfragments (TDFs) in symptomatic and asymptomatic soybean plants were carried out. Pollens in asymptomatic plants do not have defects in number, size, shape and function. However, in symptomatic plant, pollens were found nonviable, abnormalin shape and with reduced germination ability. Here, we employed a computational approach, exploring invaluable resources. The tissue-specific transcript profile of symptomatic and asymptomatic sources was compared to determine differentiallyexpressed TDFs associated with FBD to improve its basic understanding. A total of 60 decamer primers produced 197 scorable amplicons, ranged 162–1130 bp, of which 171 were monomorphic and 26 were differentially regulated. Reproducible TDFs were sequenced and characterized for their homology analysis, annotation, protein–protein interaction, subcellular localization and their physical mapping. Homology-based annotation of TDFs in soybean revealed presence of two characterized and seven uncharacterized hits. Annotation of characterized sequences showed presence of genes, namely auxin response factor 9(ARF9) and forkhead-associated (FHA) domain, which are directly involved in plant development through various pathways, such as hormonal regulation, plant morphology, embryogenesis and DNA repair.

【 授权许可】

Unknown