Functional analysis of PI-like gene in relation to flower development from bamboo (Bambusa oldhamii)

Journal of genetics

Qiaolu Zang¹  Yan Shi¹  Shinan Liu¹  Xinchun Lin1¹  Quan Shi¹  Longfei Zhu¹  Yingwu Xu¹ 

[1] The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Linan, Hangzhou 311300, People’s Republic of China$$

关键词: PISTILLATA-like gene;    C-terminal domain;    flower development;    Bambusa oldhamii.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Bamboo flowering owns many unique characteristics and remains a mystery. To investigate the molecular mechanisms underlying flower development in bamboo, a petal-identity gene was identified as a PISTILLATA homologue named BoPI from Bambusa oldhamii (bamboo family). Expression analysis showed that BoPI was highly expressed in flower organs and gradually increased during flower development stage, suggesting that BoPI played an important role in flower development. Ectopic expression of BoPI in Arabidopsis caused conversion of sepals to petals. 35S::BoPI fully rescued the defective petal formation in the pi-1 mutant. BoPI could interact with BoAP3 protein in vitro. These results suggested that BoPI regulated flower development of bamboo in a similar way with PI. Besides flower organs, BoPI was also expressed in leaf and branch, which revealed that BoPI may involve in leaf and branch development. Similar to other MIKC-type gene, BoPI contained the Cterminal sequence but its function was controversial. Ectopic expression of the C-terminal deletion construct (BoPI-𝛥C) in Arabidopsis converted sepals to petals; BoPI-𝛥C interacted with BoAP3 on yeast two-hybrid assay, just like the full-length construct. The result implied that the C-terminal sequence may not be absolutely required for organ identity function in the context of BoPI.

【 授权许可】

Unknown