Upregulation of the matrix metalloproteinase‐1 gene by the Ewing's sarcoma associated EWS‐ER81 and EWS‐Fli‐1 oncoproteins, c‐Jun and p300

FEBS Letters

Janknecht, Ralf²  Fuchs, Bruno²  Inwards, Carrie Y¹ 

[1] Department of Surgical Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA

关键词: c-Jun;    Ewing's sarcoma;    Matrix metalloproteinase-1;    p300;    Transcription;    CTD;    C-terminal domain;    EWS;    Ewing's sarcoma;    GST;    glutathione S-transferase;    MMP;    matrix metalloproteinase;    NTD;    N-terminal domain;   

DOI  :  10.1016/S0014-5793(03)00984-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanisms of action of Ewing's sarcoma (EWS) associated EWS-ETS oncoproteins have largely remained unresolved. Here, we analyzed how two EWS-ETS proteins, EWS-ER81 and EWS-Fli-1, in vitro activate the matrix metalloproteinase (MMP)-1 promoter that is upregulated in a subset of EWSs. EWS-ER81 and EWS-Fli-1 interact with and thereby activate the MMP-1 promoter, which is potentiated by the cofactor p300 and the proto-oncoprotein c-Jun. Further, EWS-ER81 binds to c-Jun in vitro and in vivo. The interaction between c-Jun, p300 and EWS-ER81 or EWS-Fli-1 may also be relevant to the regulation of other yet-to-be-identified genes that are responsible for EWS formation.

【 授权许可】

Unknown