Journal of genetics | |
Cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila | |
Champakali Ayyub11  | |
[1] Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India$$ | |
关键词: Drosophila; cullin-5; cullin-2; trachea; bouton; ovary.; | |
DOI : | |
学科分类:生物科学(综合) | |
来源: Indian Academy of Sciences | |
【 摘 要 】
Cullins confer substrate specificity to E3-ligases which are multi-protein complexes involved in ubiquitin-mediated protein degradation or modification. There are six cullin genes in Drosophila melanogaster. We have raised an antibody against Cul-5 and demonstrated that it expresses in neuronal and non-neuronal cells throughout development. In the embryonic tracheal system, Cul-5 is enriched at fusion sites together with E-Cadherin and Fasciclin III. Mutations of cul-5 do not affect tracheal development but do show defects in the organization of synaptic boutons at the larval neuromuscular junction where the protein is expressed in a subset of motoneuron terminals. Loss of function of another cullin gene ‘cul-2’ results in similar defects at the larval neuromuscular junction although cul-2;cul-5 double mutants do not show an enhanced phenotype. Both cul-2 and cul-5 mutants show similar aberrations in the development of female germ line. Our results suggest that both of these cullin proteins participate in similar developmental processes.
【 授权许可】
Unknown
【 预 览 】
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