AFLP studies on downy-mildew-resistant and downy-mildew-susceptible genotypes of opium poppy

Journal of genetics

Om P. Dhawan²  Suman P. S. Khanuja¹  Mukesh K. Dubey²  Ajit K. Shasany3¹  Ashutosh K. Shukla¹ 

[1] $$;Pps-1 and Jawahar-16 as well as$$

关键词: AFLP;    disease resistance;    DNA markers;    downy mildew;    Papaver somniferum;    Peronospora arborescens.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Downy mildew (DM) caused by Peronospora arborescens, is a serious disease in opium poppy (Papaver somniferum), which has a world-wide spread. The establishment of DM-resistant cultivars appears to be a sustainable way to control the disease. In this paper, we present the results of a study aimed at the identification of amplified fragment length polymorphism (AFLP) markers for DM-resistance in opium poppy. Three opium poppy genotypes (inbred over about 10 years): Pps-1 (DM-resistant), Jawahar-16 (DM-susceptible) and H-9 (DM-susceptible) were crossed in a diallel manner and the F1 progeny along with the parents were subjected to AFLP analysis of chloroplast (cp) and nuclear DNA with seven and nine EcoRI / MseI primer combinations, respectively. cpDNA AFLP analysis identified 24 Pps-1 (DM-resistant)-specific unique fragments that were found to be maternally inherited in both the crosses, Pps-1 × Jawahar-16 and Pps-1 × H-9. In the case of nuclear DNA AFLP analysis, it was found that 17 fragments inherited from Pps-1 were common to the reciprocal crosses of bothPps-1 and Jawahar-16 as well as

【 授权许可】

Unknown