Screening the Tomato (Lycopersicum esculentum Mill.) Lines for the Resistance to Root-knot Nematodes and Verticillium Wilt by DNA Markers

Journal of Applied Biological Sciences

Seçkin SOYA¹  Bahattin TANYOLAC¹ 

关键词: Tomato;    root-knot nematode;    Verticillium wilt;    disease resistance;    DNA markers;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Inthisstudy,itwasaimedtodeterminetheresistancetoroot-knotnematodesandVerticilliumwiltof288tomato(LycopersicumesculentumMill.)samplesbyDNAmarkers.First,itwasestablishedthatwhetherthetomatosampleshaveprimerbindingsiteornotbytheCAPSprimerswhicharespecificallydesignedforbothofthediseases.Intheanalysisofprimerbindingsitetoroot-knotnematodesandVerticilliumwiltdiseases,itisfoundthatinthe288tomatosamples,247and235tomatosampleshavespecificprimerbindingsiterespectively.Then,restrictionenzymes(TaqIandHincII)wereusedtounderstandwhethertheindividualswerehomozygoteorheterozygoteresistant,orsusceptibletothesediseasesfordeterminationofresistancetoroot-knotnematodesandVerticilliumwilt.Itisfoundthat200samplesweresusceptible,45sampleswereheterozygoteresistantand2sampleswerehomozygoteresistanttoroot-knotnematodes,and125samplesweresusceptible,110sampleswereheterozygotetoVerticilliumwilt.

【 授权许可】

Unknown