Understanding protein–protein interactions by genetic suppression

Journal of genetics

Dipankar Chatterji1¹  Sitaraman Sujatha¹ 

[1] Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India$$

关键词: genetic suppression;    protein–protein interaction;    allele specificity;    long-range interaction.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Protein–protein interactions influence many cellular processes and it is increasingly being felt that even a weak and remote interplay between two subunits of a protein or between two proteins in a complex may govern the fate of a particular biochemical pathway. In a bacterial system where the complete genome sequence is available, it is an arduous task to assign function to a large number of proteins. It is possible that many of them are peripherally associated with a cellular event and it is very difficult to probe such interaction. However, mutations in the genes that encode such proteins (primary mutations) are useful in these studies. Isolation of a suppressor or a second-site mutation that restores the phenotype abolished by the primary mutation could be an elegant yet simple way to follow a set of interacting proteins. Such a reversion site need not necessarily be geometrically close to the primary mutation site.

【 授权许可】

Unknown