Myogenic tone in mouse mesenteric arteries: evidence for P2Y receptor-mediated, Na+, K+, 2Cl− cotransport-dependent signaling

Purinergic Signalling

Georgy V. Maximov³  Pavel Hamet⁴  Svetlana V. Koltsova¹  Julie L. Lavoie⁴  Sergei N. Orlov²  Sergei V. Kotelevtsev³  Johanne Tremblay⁴  Ryszard Grygorczyk⁴ 

[1] University of Montreal Hospital Centre (CRCHUM)$$Russian Academy of Medical Sciences$$;University of Montreal Hospital Centre (CRCHUM)$$Russian Academy of Medical Sciences$$M.V. Lomonosov Moscow State University$$Centre de recherche, CRCHUM—Technopôle Angus$$;M.V. Lomonosov Moscow State University$$;University of Montreal Hospital Centre (CRCHUM)$$

关键词: Na+;    K+;    2Cl− cotransport;   

DOI  :  10.1007/s11302-009-9160-4

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

This study examines the action of agonists and antagonists of P2 receptors on mouse mesenteric artery contractions and the possible involvement of these signaling pathways in myogenic tone (MT) evoked by elevated intraluminal pressure. Both ATP and its non-hydrolyzed analog α,β-ATP triggered transient contractions that were sharply decreased in the presence of NF023, a potent antagonist of P2X₁ receptors. In contrast, UTP and UDP elicited sustained contractions which were suppressed by MRS2567, a selective antagonist of P2Y₆ receptors. Inhibition of Na⁺, K⁺, 2Cl⁻ cotransport (NKCC) with bumetanide led to attenuation of contractions in UTP- but not ATP-treated arteries. Both UTP-induced contractions and MT were suppressed by MRS2567 and bumetanide but were insensitive to NF023. These data implicate a P2Y₆-mediated, NKCC-dependent mechanism in MT of mesenteric arteries. The action of heightened intraluminal pressure on UTP release from mesenteric arteries and its role in the triggering of P2Y₆-mediated signaling should be examined further.

【 授权许可】

Unknown