Myogenic tone in mouse mesenteric arteries: evidence for P2Y receptor-mediated, Na+, K+, 2Cl− cotransport-dependent signaling

Svetlana V. Koltsova; Georgy V. Maximov; Sergei V. Kotelevtsev; Julie L. Lavoie; Johanne Tremblay; Ryszard Grygorczyk; Pavel Hamet; Sergei N. Orlov

doi:10.1007/s11302-009-9160-4

Purinergic Signalling

September 2009, Volume 5, Issue 3, pp 343–349

Myogenic tone in mouse mesenteric arteries: evidence for P2Y receptor-mediated, Na⁺, K⁺, 2Cl⁻ cotransport-dependent signaling

Authors
Authors and affiliations

Svetlana V. Koltsova
Georgy V. Maximov
Sergei V. Kotelevtsev
Julie L. Lavoie
Johanne Tremblay
Ryszard Grygorczyk
Pavel Hamet
Sergei N. OrlovEmail author

Original Paper

First Online:: 22 April 2009

Received:: 22 December 2008
Accepted:: 31 March 2009

DOI: 10.1007/s11302-009-9160-4

Cite this article as:: Koltsova, S.V., Maximov, G.V., Kotelevtsev, S.V. et al. Purinergic Signalling (2009) 5: 343. doi:10.1007/s11302-009-9160-4

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Abstract

This study examines the action of agonists and antagonists of P2 receptors on mouse mesenteric artery contractions and the possible involvement of these signaling pathways in myogenic tone (MT) evoked by elevated intraluminal pressure. Both ATP and its non-hydrolyzed analog α,β-ATP triggered transient contractions that were sharply decreased in the presence of NF023, a potent antagonist of P2X₁ receptors. In contrast, UTP and UDP elicited sustained contractions which were suppressed by MRS2567, a selective antagonist of P2Y₆ receptors. Inhibition of Na⁺, K⁺, 2Cl⁻ cotransport (NKCC) with bumetanide led to attenuation of contractions in UTP- but not ATP-treated arteries. Both UTP-induced contractions and MT were suppressed by MRS2567 and bumetanide but were insensitive to NF023. These data implicate a P2Y₆-mediated, NKCC-dependent mechanism in MT of mesenteric arteries. The action of heightened intraluminal pressure on UTP release from mesenteric arteries and its role in the triggering of P2Y₆-mediated signaling should be examined further.

Keywords

Mesenteric arteries Myogenic tone P2Y receptors Na⁺, K⁺, 2Cl⁻ cotransport

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Copyright information

Authors and Affiliations

Svetlana V. Koltsova
- 1
- 2
Georgy V. Maximov
- 3
Sergei V. Kotelevtsev
- 3
Julie L. Lavoie
- 1
Johanne Tremblay
- 1
Ryszard Grygorczyk
- 1
Pavel Hamet
- 1
Sergei N. Orlov
- 1
- 2
- 3
- 4
Email author

1.Research CentreUniversity of Montreal Hospital Centre (CRCHUM)MoscowRussia
2.Institute of General Pathology and PathophysiologyRussian Academy of Medical SciencesMoscowRussia
3.Faculty of BiologyM.V. Lomonosov Moscow State UniversityMoscowRussia
4.Centre de recherche, CRCHUM—Technopôle AngusMontrealCanada

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