Biological characterization of Bothrops marajoensis snake venom

Journal of Venom Research

Maria A da Cruz-Höfling¹  Saraguaci Hernandez-Oliveira¹  Thalita Rocha¹  Luis Ponce-Soto¹  Léa Rodrigues-Simioni¹  Márcia Gallacci¹  Maeli Dal Pai-Silva¹  Walter LG Cavalcante¹  Priscila Randazzo-Moura¹  Charlene Galbiatti¹  Sérgio Marangoni¹ 

关键词:  Marajó lancehead;    neuromuscular junction;    neurotoxicity;    myotoxicity;    presynaptic effects;   

DOI  :  

学科分类：药理学

来源: Library Publishing Media

【 摘 要 】

This study describe s the effects of Bothrops marajoensis venom (Marajó lancehead) on isolated neuromuscular preparations of chick biventer cervicis (CBC) and mouse phrenic nerve-diaphragm (PND). At low concentrations (1µg/ml for CBC and 5µg/ml for PND), the venom exhibited a neuromuscular blocking without any damaging effect on the muscle integrity. At higher concentration (20 m g/m l for PND), together with the neuromuscular blockade, there was a moderate myonecrosis. The results show differences between mammalian and avian preparations in response to venom concentration; the avian preparation was more sensitive to venom neurotoxic effect than the mammalian preparation . The possible presynaptic mechanism underlying the neuromuscular blocking effect was reinforced by the observed increase in MEPPs at the same time ( at 15min) when the facilitation of twitch tension occurred. These results indicate that the B. marajoensis venom produced neuromuscular blockade , which appeared to be presynaptic at low concentrations with a postsynaptic component at high concentrations, leading to muscle oedema . These observations demand the fractionation of the crude venom and characterization of its active components for a better understanding of its biological dynamics.

【 授权许可】

Unknown