Stability Indicating HPTLC Method for Simultaneous Estimation of Eperisone Hydrochloride and Diclofenac Sodium in Bulk and Solid Dosage Form

Eurasian Journal of Analytical Chemistry

Kalyani A. Dannak¹  Sanjay S. Pekamwar¹  Ram S. Sakhare¹ 

关键词: eperisone hydrochloride;    diclofenac sodium;    HPTLC;    forced degradation study;   

DOI  :  10.12973/ejac.2017.00167a

学科分类：分析化学

来源: Eurasian Journal of Analytical Chemistry

【 摘 要 】

The objective of present work was to develop and validate a simple, accurate, rapid and precise stability indicating HPTLC method for simultaneous estimation of Eperisone hydrochloride (EPE) and Diclofenac sodium (DICLO) in bulk and in capsule dosage form. The method employed Merck TLC plates precoated with Silica F₂₅₄. After several trials, ethyl acetate: methanol (8:2, v/v), was chosen as the mobile phase with saturation time 15 min, which showed good resolution and acceptable peak parameters. The densitometric analysis of both drugs was carried out at 266 nm. The linearity coefficient was found to be 0.999 for Eperisone hydrochloride (EPE) and 0.999 for Diclofenac sodium (DICLO). The R_f values were found to be 0.38 ± 0.02 and 0.73 ± 0.03 for EPE and DICLO. Stability study of EPE and DICLO was carried out by Forced degradation study. The developed method was successfully applied to estimate the amount of Eperisone hydrochloride and Diclofenac sodium in bulk and capsule dosage form.

【 授权许可】

Unknown