Development and Validation of a Stability Indicating RP-HPLC Method for Determination of Xanthinol Nicotinate in Bulk and Sustained Release Tablet Dosage Forms

Eurasian Journal of Analytical Chemistry

Acharjya Sasmita Kumari¹  Annapurna M. Mathrusri¹  Sagar Suman Panda¹ 

关键词: Xanthinol nicotinate;    Nicotinic acid;    Forced degradation;    RP-HPLC;   

DOI  :  10.12973/ejac.2009.00061a

学科分类：分析化学

来源: Eurasian Journal of Analytical Chemistry

【 摘 要 】

The present paper deals with the development and validation of a stability indicating reverse phase HPLC method for the determination of Xanthinol nicotinate on Hypersil ODS C18 column (250mm × 4mm,5µm) . A mobile phase consisting of methanol: 0.01M TBAHS (50:50 % v/v) was used.Doxophylline was used as the internal standard.The flow rate was 0.8ml/min..The separation was performed at room temperature.Detection was carried out at 267nm by UV detection.The developed method was statistically  validated for the linearity,accuracy,specificity,LOD and LOQ.The specificity of the method was ascertained by forced degradation studies by acid and alkali degradation,oxidation,photolysis and heat degradation.The degraded products were well separated from the analyte with significant differences in their Retention time values.Beer Law is obeyed over a concentration range of 1-400µg mL-1 and correlation coefficient was 0.9995.

【 授权许可】

Unknown