Green synthesis of Silver nanoparticles through Calotropis gigantea leaf extracts and evaluation of antibacterial activity against Vibrio alginolyticus

Nanotechnology Development

Clara Gunapoorani Sargunar¹  Vaseeharan Baskaralingam³  Jiann Chu Chen²  Yong Chin Lin² 

[1] PG & Research Dept. Zoology, Government Arts College (Autonomous), Tamil Nadu;Dept. Aquaculture, College of Life Sciences, National Taiwan Ocean University, Taiwan;Dept. Animal Health and Management, Alagappa University, Alagappa Nagar, Tamil Nadu

关键词: silver nanoparticle;    Vibrio alginolyticus;    aquaculture;    Artemia franciscana;    Calotropis gigantea.;   

DOI  :  10.4081/nd.2012.e3

学科分类：工程和技术（综合）

来源: Pagepress

【 摘 要 】

Green synthesized silver nanoparticles byCalotropis gigantealeaf extract were used to study the inhibitory activity against pathogenicVibrio alginolyticus , isolated from wildArtemia franciscanacysts. Silver nanoparticle synthesis was observed using UV-visible spectroscopy and the morphological characteristics were analyzed by atomic force microscope (AFM). In the present study, increasing concentrations of silver nanoparticles synthesized on LB agar plates effectively reduced the number of colonies ofV. alginolyticus . A decrease in colonies (CFUs) was observed at 5 mg/mL of silver nanoparticle concentration and the complete inhibition of V. alginolyticus was observed at 20 mg/mL of silver nanoparticle concentration on LB agar plates. In vivo controlling efficiency of silver nanoparticles was tested in anA. franciscanahatching system. Effective control ofV. alginolyticusin brine shrimpA. franciscanahatching units was achieved by experimental infection and treatment with silver nanoparticles. Experimental infection studies showed thatV. alginolyticusinfectedArtemianauplii treated with silver nanoparticles (10 mg/mL) had greater survival (>40%) than silver nanoparticles not treated with nauplii. Based on the findings of this study, it is recommended that low concentrations of green synthesized silver nanoparticles should be further investigated for other potential experimental models to control potential medical pathogens.

【 授权许可】

Unknown