Body composition and grip strength are improved in transgenic sickle mice fed a high-protein diet

Journal of Nutritional Science

Susanne Roser-Page¹  Gale W. Newman⁴  Hyacinth I. Hyacinth⁴  Jacqueline M. Hibbert⁴  Prasanthi Chappa³  Shayla Cue⁴  Jonathan K. Stiles⁴  M. Neale Weitzmann²  Tatyana Vikulina²  David R. Archer³  Alexander Quarshie⁴  Patrice L. Capers⁴ 

[1] Atlanta VA Medical Center;Emory University School of Medicine;Emory University;Morehouse School of Medicine

关键词: High-protein diet;    Sickle cell disease;    Grip strength;    Body composition;   

DOI  :  10.1017/jns.2014.63

学科分类：卫生学

来源: Cambridge University Press

【 摘 要 】

Using a sequential in vitro/in vivo approach, we tested the ability of botanical extracts to influence biomarkers associated with bone resorption and bone formation. Pomegranate fruit and grape seed extracts were found to exhibit anti-resorptive activity by inhibiting receptor activator of nuclear factor-κB ligand (RANKL) expression in MG-63 cells and to reduce IL-1β-stimulated calvarial 45Ca loss. A combination of pomegranate fruit and grape seed extracts were shown to be effective at inhibiting bone loss in ovariectomised rats as demonstrated by standard histomorphometry, biomechanical and bone mineral density measurements. Quercetin and licorice extract exhibited bone formation activity as measured by bone morphogenetic protein-2 (BMP-2) promoter activation, increased expression of BMP-2 mRNA and protein levels, and promotion of bone growth in cultured mouse calvariae. A combination of quercetin and licorice extract demonstrated a potential for increasing bone mineral density in an intact female rat model as compared with controls. The results from this sequential in vitro/in vivo research model yielded botanical extract formulas that demonstrate significant potential benefits for bone health.

【 授权许可】

Unknown