Over-expression of talin 1 and integrin-linked kinase in PBMCs of patients with ankylosing spondylitis: a proteomic study

Clinical and Experimental Rheumatology

Zetao Liao¹  Zhixiang Huang¹  Benrong Zheng¹  Tianwang Li¹  Huiqiong Lu¹  Like Zhao¹  Xinwei Wang¹  Jieruo Gu¹  Qu Lin¹  Zhiming Lin¹ 

关键词: ankylosing spondylitis;    two-dimensional electrophoresis;    mass spectrometry;    talin1;    integrin-linked kinase;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES: Ankylosing spondylitis (AS) is difficult to diagnose in its early stage due to the lack of simple and specific diagnostic indicators. This study was performed to screen candidate AS-associated proteins from peripheral blood mononuclear cells (PBMCs) of AS patients by combining a two-dimensional electrophoresis (2-DE) technique with mass spectrometry (MS) analysis. METHODS: Twelve subjects consisting of 6 AS patients and 6 healthy volunteers (HVs) were enrolled in the 2-DE experiments. The protein expression patterns of PBMCs from different groups were analysed by PDQuest software, and the protein spots over/under-expressed by more than 2-fold between the two groups were identified by MS analysis. Western blot analyses were used to verify the differentially expressed proteins in 32 AS patients and 32 HVs. RESULTS: Six proteins including pyruvate kinase (PK), profilin 1 (PFN1), talin 1 (TLN1), Chain A of cyclophilin A (CyPA), unknown protein (gi|16306948) and integrin-linked kinase (ILK) were identified from 10 over-expressed protein spots found by 2-DE in the AS group. Western blot experiments confirmed a higher expression of both TLN1 and ILK in AS group compared to the HV group (p

【 授权许可】

Unknown