Effects of celecoxib on human chondrocytes - enhanced production of chemokines

Clinical and Experimental Rheumatology

H. Nakamura¹  T. Kato¹  K. Yudoh¹  K. Masuko¹  K. Nishioka¹ 

关键词: Selective COX-2 inhibitor;    diclofenac;    chondrocytes;    osteoarthritis;    rheumatoid arthritis;    chemokines;    MMP;    nitric oxide.;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES: The purpose of this study was to examine the effects of a selective cyclooxigenase-2 (COX-2) inhibitor (celecoxib) comparing diclofenac. METHODS:Using chondrocytes derived from cartilage of non-arthritic (NA) subjects or patients with osteoarthritis (OA) or rheumatoid arthritis (RA), we examined the effects of celecoxib on incorporation of ³H-thymidine and ³⁵S-sulfate, apoptosis, and production of matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, and regulated upon activation, normal T cell expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1a and nitric oxide (NO).RESULTS:Celecoxib and diclofenac tended to reduce ³H-tymidine incorporation of chondrocytes. Celecoxib induced apoptosis in a dose-dependent manner, but to a lesser degree than diclofenac. Celecoxib inhibited proteoglycan synthesis (indicated by ³⁵S-sulfate incorporation) in NA chondrocytes, but not in OA and RA chondrocytes. Celecoxib increased interleukin-1 (IL-1)-induced production of RANTES and MIP-1α by chondrocytes and decreased IL-1-induced NO production by chondrocytes, whereas it did not affect MMP production.CONCLUSIONS:Celecoxib had both beneficial and adverse effects on chondrocytes. RA, OA and NA chondrocytes showed different responses. Interestingly, celecoxib enhanced the production of chemokines.

【 授权许可】

Unknown