Regulation of the selenoprotein SelS by glucose deprivation and endoplasmic reticulum stress – SelS is a novel glucose‐regulated protein

FEBS Letters

Bolton, Kristy¹  Feng, Helen C¹  Gao, Yuan¹  Collier, Greg R¹  Bishara, Natalie¹  Sunderland, Terry¹  Walder, Ken¹  Kantham, Lakshmi¹  Quick, Melissa¹ 

[1] Metabolic Research Unit, School of Health Sciences, Deakin University, Pigdons Road, Waurn Ponds, Vic. 3217, Australia

关键词: Selenoprotein;    Glucose-regulated protein;    Glucose deprivation;    Endoplasmic reticulum stress;    Gene expression;    DMEM;    Dulbecco's modified Eagle's medium;    ER;    endoplasmic reticulum;    ERSE;    ER stress response element;    FBS;    fetal bovine serum;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    GFP;    green fluorescent protein;    GRP;    glucose-regulated protein;    HO-1;    heme oxygenase 1;    MTT;    3-(4;    5-dimethylthiazol-2-yl)-2;    5-diphenyl tetrazolium bromide;    RT;    reverse transcription;    Tm;    tunicamycin;    Tg;    thapsigargin;   

DOI  :  10.1016/S0014-5793(04)00296-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

SelS is a newly identified selenoprotein and its gene expression is up-regulated in the liver of Psammomys obesus after fasting. We have examined whether SelS is regulated by glucose deprivation and endoplasmic reticulum (ER) stress in HepG2 cells. Glucose deprivation and the ER stress inducers tunicamycin and thapsigargin increased SelS gene expression and protein content several-fold in parallel with glucose-regulated protein 78. The overexpression of SelS increased Min6 cell resistance to oxidative stress-induced toxicity. These results indicate that SelS is a novel member of the glucose-regulated protein family and its function is related to the regulation of cellular redox balance.

【 授权许可】

Unknown