Increased state 4 mitochondrial respiration and swelling in early post‐ischemic reperfusion of rat heart

FEBS Letters

Solaini, G²  Baracca, A¹  Bosetti, F²  Lenaz, G¹ 

[1] Dipartimento di Biochimica ‘G. Moruzzi’, Università di Bologna, Bologna, Italy;Scuola Superiore di Studi Universitari e di Perfezionamento S. Anna, Piazza dei Martiri 33, 56127 Pisa, Italy

关键词: Mitochondrion;    Ischemia and reperfusion;    Respiration;    Uncoupling;    Heart;   

DOI  :  10.1016/S0014-5793(04)00294-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolated rat hearts were exposed to 30 min ischemia or to 30 min ischemia followed by 2, 5 or 40 min reperfusion and mitochondria were isolated at these different time points. ADP-stimulated, succinate-dependent respiration rate (state 3) was not significantly changed at the different time points examined. In contrast, state 4 (non-ADP-stimulated) respiration rate was significantly increased after 30 min ischemia, and it increased further during the first post-ischemic reperfusion period. Mitochondrial swelling, as evaluated under conditions of the major controlled ion channels (i.e. permeability transition pore and ATP-dependent mitochondrial K⁺ channel) closed, significantly increased in parallel. It is suggested that the inner mitochondrial membrane permeability is increased under exposure of the heart to ischemia and early reperfusion, and that the phenomenon is reversible upon subsequent long periods of reperfusion.

【 授权许可】

Unknown