Stimulation of respiration by methylene blue in rat liver mitochondria

FEBS Letters

Visarius, Theresa M¹  Lauterburg, Bernhard H¹  Stucki, Jörg W² 

[1] Department of Clinical Pharmacology, University of Berne, Murtenstrasse 35, 3010 Berne, Switzerland;Department of Pharmacology, University of Berne, 3010 Berne, Switzerland

关键词: Mitochondrion;    Oxidative Phosphorylation;    Uncoupling;    Methylene Blue;    Ifosfamide;    Chloroacetaldehyde;   

DOI  :  10.1016/S0014-5793(97)00767-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of methylene blue on isolated rat liver mitochondria in the presence and absence of chloroacetaldehyde was investigated. Fatty acid oxidation was inhibited by chloroacetaldehyde and subsequently stimulated by methylene blue. Assessment of tightly coupled mitochondria revealed decreasing respiratory control ratios induced by increasing concentrations of methylene blue and methylene blue provoked mitochondrial swelling. In uncoupled mitochondria, methylene blue promoted a concentration-dependent stimulation of respiration. These findings provide evidence that methylene blue, the redox dye currently used as an antidote for encephalopathy associated with alkylating chemotherapy, uncouples oxidative phosphorylation and acts as an electron transfer mediator to stimulate mitochondrial respiration.

【 授权许可】

Unknown