Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells

FEBS Letters

Lee, Jeung Hwa²  Lee, Sun Joo¹  Chun, Jang-Soo¹  Jeon, Hong Bae¹  Yoo, Yung Joon¹  Yoo, Jong Shin² 

[1] Department of Life Science, Kwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju 500-712, South Korea;Korea Basic Science Institute, Daejun 305-333, South Korea

关键词: Chondrocyte;    Chondrogenesis;    Extracellular matrix;    Mesenchymal cell;    Signaling pathway;    AKR;    aldo-keto reductase;    CA-II;    carbonic anhydrase-II;    CRABP;    cellular retinoic acid binding protein;    ECM;    extracellular matrix;    FABP;    fatty acid binding protein;    PAPS;    3′-phosphoadenosine 5′-phosphosulfate;    PMF;    peptide mass fingerprinting;   

DOI  :  10.1016/S0014-5793(04)00243-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.

【 授权许可】

Unknown