Molecular cloning and characterization of β1,4‐N‐acetylgalactosaminyltransferases IV synthesizing N,N′‐diacetyllactosediamine1

FEBS Letters

Kameyama, Akihiko²  Kwon, Yeon-Dae²  Narimatsu, Hisashi²  Iwai, Toshie²  Ishizuka, Yasuko²  Nakanishi, Hiroshi¹  Kikuchi, Norihiro²  Sato, Takashi²  Gotoh, Masanori²  Kiyohara, Katsue² 

[1]Biological Information Research Center, AIST, Central-6, 1-1 Higashi, Tsukuba, Ibaraki 305-8568, Japan

[2]Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Open Space Laboratory Central-2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan

关键词: Glycosyltransferase;    N-Acetylgalactosaminyltransferase;    N-Glycan;    N;    N′-Diacetyllactosediamine;    Glycodelin;    Lutropin;    LacdiNAc;    N;    N′-diacetyllactosediamine (GalNAcβ1-4GlcNAc);    GalNAc;    N-acetylgalactosamine;    GlcNAc;    N-acetylglucosamine;    LH;    lutropin;    Man;    mannose;    Gd;    glycodelin;    NeuAc;    N-acetylneuraminic acid;    Gal;    galactose;    Le;    Lewis;    Lex;    Galβ1-4(Fucα1-3)GlcNAc;    Fuc;    fucose;    Ley;    Fucα1-2Galβ1-4(Fucα1-3)GlcNAc;    β4GalNAc-T;    β1;    4-N-acetylgalactosaminyltransferase;    β4GT;    β1;    4-glycosyltransferase;    EST;    expressed sequence tag;    ORF;    open reading frame;    RACE;    rapid amplification of cDNA ends;    HEK;    human embryonic kidney;    GlcA;    glucuronic acid;    pNp;    para-nitrophenyl;    Bz;    benzyl;    Glc;    glucose;    Xyl;    xylose;    MS;    mass spectrometry;    MALDI-TOF;    matrix-assisted laser desorption/ionization-time of flight;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/S0014-5793(04)00219-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A sequence highly homologous to β1,4-N-acetylgalactosaminyltransferase III (β4GalNAc-T3) was found in a database of human expressed sequence tags. The full-length open reading frame of the gene, β4GalNAc-T4 (GenBank accession number AB089939), was cloned using the 5′ rapid amplification of cDNA ends method. It encodes a typical type II transmembrane protein of 1039 amino acids having 42.6% identity with β4GalNAc-T3. The recombinant enzyme transferred N-acetylgalactosamine to N-acetylglucosamine-β-benzyl with a β1,4-linkage to form N,N′-diacetyllactosediamine as did β4GalNAc-T3. In specificity toward oligosaccharide acceptor substrates, it was quite similar to β4GalNAc-T3 in vitro, however, the tissue distributions of the two enzymes were quite different. These results indicated that the two enzymes have similar roles in different tissues.

【 授权许可】

Unknown