Generation of Arabidopsis thaliana plants with complex N‐glycans lacking β1,2‐linked xylose and core α1,3‐linked fucose

FEBS Letters

Steinkellner, H.¹  Strasser, R.¹  Altmann, F.²  Glössl, J.¹  Mach, L.¹ 

[1] Institut für Angewandte Genetik und Zellbiologie, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Wien, Austria;Institut für Chemie, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Wien, Austria

关键词: β1;    2-Xylosyltransferase;    Core α1;    3-fucosyltransferase;    Glycosyltransferase;    N-Glycan;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(04)00150-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The plant glycosyltransferases, β1,2-xylosyltransferase (XylT) and core α1,3-fucosyltransferase (FucT), are responsible for the transfer of β1,2-linked xylose and core α1,3-linked fucose residues to glycoprotein N-glycans. These glycan epitopes are not present in humans and thus may cause immunological responses, which represent a limitation for the therapeutic use of recombinant mammalian glycoproteins produced in transgenic plants. Here we report the genetic modification of the N-glycosylation pathway in Arabidopsis thaliana plants. Knockout plants were generated with complete deficiency of XylT and FucT. These plants lack antigenic protein-bound N-glycans and instead synthesise predominantly structures with two terminal βN-acetylglucosamine residues (GlcNAc₂Man₃GlcNAc₂).

【 授权许可】

Unknown