Rev‐erbα upregulates NF‐κB‐responsive genes in vascular smooth muscle cells

FEBS Letters

Migita, Hideyuki²  Kawai, Kohichi²  Morser, John¹ 

[1] Cardiovascular Research, Berlex Biosciences, Richmond, CA 94804, USA;Cardiovascular Research, Drug Discovery Institute, Nihon Schering K.K., 1900-1, Togo Mobara, Chiba 297-0017, Japan

关键词: Nuclear receptor;    Nuclear factor-κB;    Interleukin-6;    Cyclooxygenase-2;    Vascular smooth muscle cell;    COX-2;    cyclooxygenase-2;    HUVECs;    human umbilical vein endothelial cells;    IL;    interleukin;    NF-κB;    nuclear factor-κB;    RORα;    retinoic acid receptor-related orphan receptor-α;    RORE;    ROR response element;    PPAR;    peroxisome proliferator-activated receptor;    rRNA;    ribosomal RNA;    TNF-α;    tumor necrosis factor-α;    VSMCs;    vascular smooth muscle cells;   

DOI  :  10.1016/S0014-5793(04)00118-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rev-erbα and retinoic acid receptor-related orphan receptor-α (RORα) are orphan nuclear receptors but their effects on transcription are opposed. Here, we show that Rev-erbα was expressed predominantly in vascular smooth muscle cells (VSMCs) rather than endothelial cells. Overexpression of Rev-erbα upregulated the expression of interleukin-6 and cyclooxygenase-2, and increased transactivation by NF-κB and translocation of p65 to the nucleus in A7r5 VSMCs. Furthermore, the expression of Rev-erbα was upregulated by RORα1 but that upregulation was attenuated by Rev-erbα itself in A7r5 VSMCs. These results suggest a regulatory link between Rev-erbα and the NF-κB pathway.

【 授权许可】

Unknown