期刊论文详细信息
FEBS Letters
Induction of 1,N 2‐etheno‐2′‐deoxyguanosine in DNA exposed to β‐carotene oxidation products
Di Mascio, Paolo1  Marques, Sabrina A1  Medeiros, Marisa H.G1  Gomes, Osmar F1  Garcia, Camila C.M1  Loureiro, Ana Paula M1 
[1] Departamento de Bioquı́mica, Instituto de Quı́mica, Universidade de São Paulo, CP 26.077, CEP 05599-970 São Paulo, Brazil
关键词: β-Carotene;    β-Apo-8′-carotenal;    1;    N 2-Etheno-2′-deoxyguanosine;    Etheno adduct;    8-Oxo-7;    8-dihydro-2′-deoxyguanosine;    DNA damage;    Retinal;    βC;    β-carotene;    1;    N 2-ϵdGuo;    1;    N 2-etheno-2′-deoxyguanosine;    dGuo;    2′-deoxyguanosine;    ϵdAdo;    1;    N 6-etheno-2′-deoxyadenosine;    N 2;    3-ϵdGuo;    N 2;    3-etheno-2′-deoxyguanosine;    N 2;    3-ϵGua;    N 2;    3-etheno-guanine;    1;    N 2-ϵGua;    1;    N 2-etheno-guanine;    8-oxodGuo;    8-oxo-7;    8-dihydro-2′-deoxyguanosine;    HPLC;    high performance liquid chromatography;    ESI/MS;    electrospray ionization mass spectrometry;    LC/ESI/MS-MS;    liquid chromatography/electrospray ionization tandem mass spectrometry;    MRM;    multiple reaction monitoring;   
DOI  :  10.1016/S0014-5793(04)00084-5
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
PDF
【 摘 要 】

Epidemiological studies testing the effect of β-carotene in humans have found a relative risk for lung cancer in smokers supplemented with β-carotene. We investigated the reactions of retinal and β-apo-8′-carotenal, two β-carotene oxidation products, with 2′-deoxyguanosine to evaluate their DNA damaging potential. A known mutagenic adduct, 1,N 2-etheno-2′-deoxyguanosine, was isolated and characterized on the basis of its spectroscopic features. After treatment of calf thymus DNA with β-carotene or β-carotene oxidation products, significantly increased levels of 1,N 2-etheno-2′-deoxyguanosine and 8-oxo-7,8-dihydro-2′-deoxyguanosine were quantified in DNA. These lesions are believed to be important in the development of human cancers. The results reported here may contribute toward an understanding of the biological effects of β-carotene oxidation products.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201912020313890ZK.pdf 690KB PDF download
  文献评价指标  
  下载次数:14次 浏览次数:32次