Antiviral activity of caspase inhibitors: effect on picornaviral 2A proteinase

FEBS Letters

Roetzer, Andreas¹  Kuechler, Ernst¹  Seipelt, Joachim¹  Deszcz, Luiza¹  Vassilieva, Elena¹ 

[1] Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Medical Biochemistry, Division of Biochemistry, University of Vienna, Dr. Bohr Gasse 9/3, A-1030 Vienna, Austria

关键词: Rhinovirus;    Picornavirus;    2A proteinase;    eIF4GI cleavage;    Caspase inhibitor;    Apoptosis;    HRV2;    human rhinovirus serotype 2;    CV;    coxsackievirus;    2Apro;    2A proteinase;    pNA;    para-nitroanilide;    eIF;    eukaryotic initiation factor;    zVAD.fmk;    benzyloxycarbonyl-Val-Ala-Asp(OMe) fluoromethyl ketone;    zIETD.fmk;    benzyloxycarbonyl-Ile-Glu(OMe)-Thr-Asp (OMe) fluoromethyl ketone;    SDS–PAGE;    sodium dodecyl sulfate–polyacrylamide gel electrophoresis;    p.i.;    post infection;    TCID50;    50% tissue culture infectious dose;   

DOI  :  10.1016/S0014-5793(04)00069-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peptide-based fluoromethyl ketones have been considered for many years to be highly specific caspase inhibitors distinctly blocking the progress of apoptosis in a variety of systems. Here we demonstrate that these compounds can significantly reduce rhinovirus multiplication in cell culture. In their methylated forms they block eIF4GI cleavage in vivo and in vitro and inhibit the activity of picornaviral 2A proteinases.

【 授权许可】

Unknown