Antibodies against EMC virus RNA‐VPg recognize Tyr‐(5′P→O)‐pU and immunostain infected cells

FEBS Letters

Bordunova, Olga A¹  Shatskaya, Galina S¹  Drygin, Yuri F¹  Nadezhdina, Elena S³  Turina, Oksana V¹  Veiko, Vladimir P² 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Khokhlov st., 119899 Moscow, Russia;Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia;Institute of Protein Research, Pushchino, Moscow Region, Russia

关键词: Picornavirus;    Encephalomyocarditis virus;    RNA-protein covalent complex;    Ribotope;    Membrane immunochemical analysis;    Immunofluorescent microscopy;    Ab;    antibody;    BSA;    bovine serum albumin;    EMC;    encephalomyocarditis;    CHO;    Chinese hamster ovary;    CLU;    covalent linkage unit;    pfu;    plaque-forming unit;    PAG;    polyacrylamide gel;    RP HPLC;    reverse phase high-performance liquid chromatography;    TLC;    thin layer chromatography;   

DOI  :  10.1016/S0014-5793(97)01601-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Covalent complexes of nucleic acids and proteins are widespread among viruses. Covalent complexes of RNA and proteins are proposed to exist in eukaryotic cells. The goal of this work was to obtain specific antibodies to the covalent linkage unit (CLU) between virus RNA and protein to search cellular RNA-protein complexes. Antibodies were generated by direct immunization of a rabbit with the BSA-coupled EMC virus RNA-VPg complex. By a dot-blot immunoassay and immunofluorescent microscopy it was found that the antibodies specifically recognize both EMC virus RNA-VPg and synthetic CLU-containing compounds. Thus, a fraction of the antibodies was directed to CLU.

【 授权许可】

Unknown