Prion protein is a component of the multimolecular signaling complex involved in T cell activation

FEBS Letters

Garofalo, Tina²  Pavan, Antonio¹  Sorice, Maurizio²  Lucania, Giuseppe²  Circella, Annapia²  Manganelli, Valeria²  Mattei, Vincenzo²  Misasi, Roberta² 

[1] Dipartimento di Medicina Sperimentale, Università di L’Aquila, Via Vetoio Coppito 2, 67100 L’Aquila, Italy;Dipartimento di Medicina Sperimentale e Patologia, Università ‘La Sapienza’, viale Regina Elena 324, 00161 Rome, Italy

关键词: Prion protein;    Lipid domain;    Raft;    Ganglioside;    T cell activation;   

DOI  :  10.1016/S0014-5793(04)00029-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study we analyzed the interaction of prion protein PrP^C with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP^C was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP^C is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrP^C and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immunoprecipitated by anti-PrP after T cell activation. These results demonstrate that PrP^C interacts with ZAP-70, suggesting that PrP^C is a component of the multimolecular signaling complex within microdomains involved in T cell activation.

【 授权许可】

Unknown