Towards the in vitro reconstitution of caveolae. Asymmetric incorporation of glycosylphosphatidylinositol (GPI) and gangliosides into liposomal membranes

FEBS Letters

Alonso, Alicia¹  Brodbeck, Urs³  Goñi, Félix M.¹  Pañeda, Covadonga²  Villar, Ana-Victoria¹  Varela-Nieto, Isabel² 

[1] Unidad de Biofı́sica (CSIC-UPV/EHU), and Departamento de Bioquı́mica, Universidad del Paı́s Vasco, Aptdo. 644, 48080 Bilbao, Spain;Instituto de Investigaciones Biomédicas ‘Alberto Sols’, CSIC-UAM, Arturo Duperier 4, 28029 Madrid, Spain;University of Berne, Institute for Biochemistry and Molecular Biology, Bühlstrasse 28, CH-3012 Berne, Switzerland

关键词: Raft;    Caveola;    Asymmetry;    Glycosylphosphatidylinositol;    Ganglioside;    Phospholipase;   

DOI  :  10.1016/S0014-5793(99)01010-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Large unilamellar vesicles consisting of phospholipids with or without cholesterol have been prepared containing GPI and/or gangliosides asymmetrically located in the outer leaflet of the bilayer. Such asymmetric distribution of GPI and gangliosides is found in ‘rafts’ and caveolae. Using these vesicles, GPI can be readily hydrolysed by phospholipases. Both cholesterol and ganglioside are seen to inhibit, in an additive way, the hydrolytic activity of GPI-specific phospholipase D.

【 授权许可】

Unknown