Involvement of caveolin‐2 in caveolar biogenesis in MDCK cells

FEBS Letters

Honsho, Masanori¹  Simons, Kai¹  Parton, Robert G.²  Lahtinen, Ulla¹  Verkade, Paul¹ 

[1] Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany;Institute for Molecular Bioscience, University of Queensland, Centre for Microscopy and Microanalysis, and School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia

关键词: Caveolin-1;    Caveolin-2;    Caveola;    MDCK;   

DOI  :  10.1016/S0014-5793(03)00135-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells caveolin-1 and -2 are found together on basolateral caveolae whereas the apical membrane, where only caveolin-1 is present, lacks caveolae. Expression of a caveolin mutant prevented the formation of the large caveolin-1/-2 hetero-oligomeric complexes, and led to intracellular retention of caveolin-2 and disappearance of caveolae from the basolateral membrane. Correspondingly, in MDCK cells over-expressing caveolin-2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin-2 in caveolar biogenesis.

【 授权许可】

Unknown