Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid‐specific promoter using in vivo electroporation

FEBS Letters

Nishimune, Yoshitake¹  Ohta, Hiroshi¹  Ike, Akiko¹  Onishi, Masayoshi¹  Nozaki, Masami¹  Iguchi, Naoko¹ 

[1] Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1, Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: Haploid gene expression;    Mouse;    Testis;    In vivo electroporation;    Promoter;   

DOI  :  10.1016/S0014-5793(04)00065-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The testicular isoform of the ornithine decarboxylase antizyme (OAZt) gene is expressed exclusively in the haploid spermatids of mice. The 357-bp region, which includes a TATA-less promoter and an untranslated region, is sufficient for OAZt gene expression in the spermatids of transgenic mice. In this study, in vivo transient transfection to living mouse testes was used to define the transcriptional regulatory elements of the OAZt gene promoter. We found that the 10-bp element that contains an initiator (Inr) plays a central role as the core promoter, in combination with a downstream element, while two cyclic adenosine monophosphate-responsive element (CRE)-like sites in the upstream region also contribute to promoter activity. The electrophoretic mobility shift assay showed binding of the testis-specific factors to these elements. Our results show that the in vivo DNA transfer technique enables detailed analysis of haploid germ cell-specific gene regulation in mice.

【 授权许可】

Unknown